Characterization of methylphosphonate as a 31P NMR pH indicator.

Phenylphosphonate: a 31P-NMR indicator of extracellular pH and volume in the isolated perfused rabbit bladder.

31P-NMR has been used extensively to estimate intracellular pH. It also can be used to measure extracellular pH and volume when an NMR-detectable extracellular phosphorous probe is used. Phosphonic acids have been suggested as useful 31P-NMR extracellular markers. The present study was designed to assess the utility of phenylphosphonic acid (PPA) as a 31P-NMR extracellular marker in perfused sm...

Effects of divalent cations and pH on phosphatidylserine model membranes: a 31P NMR study.

Received December 5,1979 SUMMARY The influence of divalent cations, and pH on the behaviour of phosphatidylserine, derived from egg phosphatidylcholine, has been examined employing 31P-NMR techniques. The addition of Ca2+ results in the observation of a "rigid lattice" 31P-NMR spectra and more than an order of magnitude increase in the spin-lattice relaxation time Tl. specific headgroup immobil...

Quantitative analysis of phospholipids by 31P-NMR.

High-field 31P nuclear magnetic resonance spectroscopy was used to quantitate phospholipids in mixtures in organic solvents. The sample is dissolved in chloroform-methanol and analyzed at 161.7 MHz with decoupling of the protons. Signals were identified using authentic compounds, and their relative distribution was measured in mole percent. The method has good accuracy and reproducibility, and ...

31P NMR of phospholipid glycerol phosphodiester residues.

Saponification of extracted tissue phospholipids yields a set of isolated glycerol 3-phosphoryl phospholipid polar headgroups from which semi-quantitative 31P NMR spectra can be obtained. The resonance signals from these molecules, which frequently have been reported as uncharacterized phosphate signals observed in perchloric acid extracts of tissue, can be used as an aid in the characterizatio...

31P NMR studies on kidney intracellular pH in acute renal acidosis.